mouse lrp6 (R&D Systems)

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R&D Systems mouse lrp6
Mouse Lrp6, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+lrp6/Mouse+LRP-6+(NP_032540)+VersaClone+cDNA/pm39443289-60-19-21
Average 92 stars, based on 1 article reviews

mouse lrp6 - by Bioz Stars, 2026-10

92/100 stars

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Incubation:

Article Title: Mapping the sclerostin– LRP4 binding interface identifies critical interaction hotspots in loops 1 and 3 of sclerostin
Article Snippet: Data analysis was performed using flowjo software (BD, Ashland, OR, USA). .. For library sorting, the cells were incubated with 5 n m soluble His‐tagged human LRP4 or 650 n m soluble His‐tagged mouse LRP6 (R&D Systems, Minneapolis, MN, USA) and 1 : 100 affinity‐purified chicken anti‐c‐Myc antibody (Immunology Consultants Laboratory, Inc., Portland, OR, USA) for 1 h at room temperature, followed by double staining with a 1 : 50 FITC‐conjugated anti‐6 × His‐tag monoclonal antibody (Invitrogen, Waltham, MA, USA) and 1 : 50 Alexa FluorTM 555‐labeled goat anti‐chicken IgY (H + L) antibody (Invitrogen) for 20 min in the dark on ice. .. The desired populations were collected on an iCyt Synergy FACS apparatus (Sony Biotechnology, San Jose, CA, USA) and allowed to recover at 30 °C with shaking at 300 rpm in SDCAA medium until the culture reached an OD 600 of 8.

Article Title: Competitive blocking of LRP4-sclerostin binding interface strongly promotes bone anabolic functions.
Article Snippet: Induction of bone formation by Wnt ligands is inhibited when sclerostin (Scl), an osteocyte-produced antagonist, binds to its receptors, the low-density lipoprotein receptor-related proteins 5 or 6 (LRP5/6).. Recently, it was shown that enhanced inhibition is achieved by Scl binding to the co-receptor LRP4.. However, it is not clear if the binding of Scl to LRP4 facilitates Scl binding to LRP5/6 or inhibits the Wnt pathway in an LRP5/6-independent manner.

Article Title: Competitive blocking of LRP4–sclerostin binding interface strongly promotes bone anabolic functions
Article Snippet: The expression of displayed proteins was detected with 1:50 mouse anti-c-Myc antibody, 9E10 (Abcam, Cambridge, MA, USA) followed by 1:50 anti-mouse IgG (whole molecule)—R-phycoerythrin antibody produced in goat (Sigma-Aldrich). .. For binding analysis of displayed Scl WT or Scl N93A , cells were incubated with different concentrations of either soluble His-tagged mouse LRP6 (R&D Systems) or with soluble His-tagged human LRP4 (R&D Systems) for 2 h at room temperature, following detection with a 1:50 FITC-conjugated anti-6xHis-tag monoclonal antibody (Invitrogen). .. For competition binding between LRP4 and LRP6, cells displaying Scl were pre-incubated with soluble LRP4 (1, 5 or 200 nM) for 2 h, followed by incubation with 200 nM soluble human LRP6-Fc (R&D Systems) for 2 h and detection with a 1:50 monoclonal anti-human IgG (Fc specific)—FITC antibody produced in mouse (Sigma Aldrich).

Article Title: Mapping the sclerostin-LRP4 binding interface identifies critical interaction hotspots in loops 1 and 3 of sclerostin.
Article Snippet: Data analysis was performed using FLOWJO software (BD, Ashland, OR, USA). .. For library sorting, the cells were incubated with 5 nM soluble His-tagged human LRP4 or 650 nM soluble His-tagged mouse LRP6 (R&D Systems, Minneapolis, MN, USA) and 1 : 100 affinity-purified chicken anti-c-Myc antibody (Immunology Consultants Laboratory, Inc., Portland, OR, USA) for 1 h at room temperature, followed by double staining with a 1 : 50 FITC-conjugated anti-6 ×His-tag monoclonal antibody (Invitrogen, Waltham, MA, USA) and 1 : 50 Alexa FluorTM 555-labeled goat anti-chicken IgY (H +L) antibody (Invitrogen) for 20 min in the dark on ice. .. The desired populations were collected on an iCyt Synergy FACS apparatus (Sony Biotechnology, San Jose, CA, USA) and allowed to recover at 30 °C with shaking at 300 rpm in SDCAA medium until the culture reached an OD600 of 8.

Affinity Purification:

Double Staining:

Purification:

Article Title: Bispecific binding agents
Article Snippet: Dose-response curves were generated by serially diluting parental and DVD-Ig proteins in assay buffer (40 mM HEPES pH 7.5, 100 mM NaCl, 1 mM CaCl2, 0.1% BSA, 0.05% Tween-20) in a 384-well Greiner microtiter plate. .. A tracer amount of in-house purified and chemically biotinylated recombinant human or rat Sclerostin (up to 1.5 nM) was added to the microtiter plate followed by the addition of a mixture containing either mouse LRP6-his or rhLRP6-Fc (R&D Systems) (up to 6-12 nM) and AlphaScreen ‘donor’ streptavidin and ‘acceptor’ protein A beads (10 mg/ml each) (PerkinElmer). ..

Recombinant:

Article Title: Haemorrhagic snake venom metalloproteases and human ADAMs cleave LRP5/6, which disrupts cell-cell adhesions in vitro and induces haemorrhage in vivo.
Article Snippet: In brief, crude venom from Crotalus atrox (Sigma-Aldrich, St Louis, MO, USA) was subjected to hydroxyapatite chromatography and anion ion-exchange chromatography. .. Recombinant human LRP6 with an Fc-tag, human ADAM8 and ADAM12, VEcadherin, and mouse LRP6 and LRP5 having a His-tag were purchased from R&D Systems, Inc. (Minneapolis, MN, USA). .. Human umbilical vein endothelial cells (HUVECs) were purchased from Becton, Dickinson and Company (Franklin Lakes, NJ, USA).